Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...
Until recently, PCR was the only method for direct isolation of desirable chromosomal segments from complex genomes. Although it is widely used for the analysis of genomes, PCR has one major ...
Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...
While the traditional cloning workflow has been used in laboratories for decades, new techniques have emerged that allow for rapid assembly of multiple genetic fragments. The Electra Vector System, ...