A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends …

DNA cloning involves creating identical copies of a specific gene using restriction enzymes to cut the desired gene, pasting it into a plasmid, and inserting it into bacteria like E. coli. The …

See the article on restriction enzymes and DNA ligase for a more concrete example of how and why these different ligation products can form.

A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site. Many restriction enzymes produce cut ends with …

For instance, the DNA may be visualized by gel electrophoresis, sent for sequencing, or digested with restriction enzymes and cloned into a plasmid. PCR is used in many research labs, and it …

For your first question: You will do PCR of the entire sample. Then a restriction enzyme is used to cut the part you want. One restriction enzyme will make the cut on the same place in all …

Thus, when they figured out what enzyme is causing that, they basically called it a restriction enzyme, 'cause it restricted the growth of viruses. Now, in order for the restriction enzyme to …

Thanks to these amino acids, an enzyme's active site is uniquely suited to bind to a particular target—the enzyme's substrate or substrates—and help them undergo a chemical reaction.

This enzyme prevents the DNA double helix ahead of the replication fork from getting too tightly wound as the DNA is opened up. It acts by making temporary nicks in the helix to release the …

Zoom into the world of biomolecules as you explore the structure and function of proteins, nucleic acids, carbohydrates, lipids, ATP, and more! Explore some of the ways that these molecules …